EFFECTS OF CONTUSION AND EXHAUSTIVE EXERCISE ON MURF1 AND MAFBX IN THE SKELETAL MUSCLE OF RATS

ABSTRACT Objective To study the effects of contusion and exhaustive exercise on expression degradation-related factors MuRF1 MAFbx in skeletal muscle rats describe repair mechanism injury. Methods Forty-two male SD were randomly divided into 7 groups. The each group killed at different time points (0h, 24h, 48h) after (E0, E24, E48) (D0, D24, D48), respectively, resting state control (C). right gastrocnemius muscles resected two parts, one for mRNAs by real-time PCR, other protein measurement Western blotting. Results Compared with group, mRNA E0 was markedly increased (P <0.05) followed a downward trend E24 E48 On hand, D24 significantly upregulated <0.01), then decreased D48 <0.01). Meanwhile, compared C gene continued to be <0.05), but (p<0.01). NF-κB contents D0, groups, as well downregulated also remarkably <0.05). Conclusion may negatively regulate process degradation / signal pathway. Level evidence III; Therapeutic studies investigating results treatment.